Intracytoplasmic sperm injection (microinjection)

Intracytoplasmic sperm injection (ICSI) is a method of in vitro fertilization,
under a microscope, we use a special technique in which we inject the sperm directly into the oocyte (egg) with a needle.
is an in vitro fertilization technique. ICSI (microinjection) is nowadays available for both men and women.
in the treatment of infertility (infertility).

In conventional IVF treatment, a large number of sperm cells are taken from the man and collected in the laboratory.
spermatozoa are placed around an egg cell taken from a woman in an environment
one of them is expected to fertilize the egg spontaneously. What happens in classical in vitro fertilization
the only difference between fertilization and normal fertilization is that fertilization takes place in a laboratory, not in the mother’s body.
In the microinjection (ICSI) method, one of the highest quality sperms from the man
is selected. It is injected into the egg cell under a microscope. So the sperm is injected into the egg
cell itself, it is inserted directly into the egg cell in the laboratory.
fertilization is achieved.

Microinjection (ICSI) is often used to increase the success rate in IVF treatment.
It is a preferred practice, and the patient groups that are especially used are mainly these:
●Male-caused infertility (infertility): ICSI is the most successful treatment for male infertility.
If the sperm count (azoospermia), motility or normal sperm percentage (morphology) is too high
low, ICSI (microinjection) can be used with almost any type of sperm.
we can achieve fertilization.
If fertilization failed in the previous IVF treatment, this time ICSI
(microinjection) technique.
ICSI in case of structural problems in the eggs and no fertilization
(microinjection) to achieve fertilization.
Sperm antibodies: In some cases, the woman’s blood contains antibodies against sperm cells, called antibodies.
substances called antibodies can be seen. These antibodies help the sperm cell to fertilize the egg cell.
prevents it. In such cases, microinjection therapy (ICSI) may be beneficial.
If sperm had to be removed directly from the testicle or epididymal tissue by operative methods (
TESE, MESA, PESA etc. procedures) fertilization is achieved by ICSI (microinjection).
Unexplained infertility (infertility) where all examinations and investigations appear normal
patients can also use the microinjection technique (ICSI).

1- Obtaining sperm – Sperm can be naturally extracted from the ejaculate (semen). Sperm in ejaculate
absence (azoospermia) or ejaculatory dysfunction, TESE (testicular
sperm extraction), MESA (Microsurgical epididymal sperm aspiration), PESA
(percutaneous epididymal sperm aspiration) techniques to remove sperm from the patient with a minimal operation.
2- Sperm selection – The sperm samples obtained are subjected to certain procedures such as centrifugation before ICSI.
processed to select a normal, live sperm for injection.
3- Egg collection and preparation – Eggs are retrieved from the woman in a tiny operation
With the enzyme hyaluronidase, the cells surrounding the egg, called cumulus, are removed.
Eggs in a phase suitable for fertilization are selected.
4- Injection of sperm into the egg – First, the egg is fixed with a tiny pipette.
Then the sperm is injected into the egg by passing through the egg membrane with a tiny injection pipette.
cell and the process is completed. This fertilization process is called microinjection (ICSI)
technique takes 10-20 minutes.
The embryo formed by the microinjection (ICSI) procedure is kept in an incubator for 2-3 days.
implanted in the woman’s uterus and followed by waiting for it to implant in the uterus and grow in a healthy way
It is done.

Fertilization is more likely to be successful with the ICSI (microinjection) technique.
Fertilization can be successful even if only one sperm is collected from the father-to-be.

The fertilization rate after ICSI (microinjection) is around 50-80 percent. Microinjection
(ICSI) has a higher success rate in fertilization and pregnancy than conventional IVF.
Sperm and egg quality and the age of the mother are very important for successful fertilization.
It is important that the best quality sperm and eggs are selected for microinjection (ICSI). Some
in some cases, fertilization may not occur even if the sperm is implanted into the egg without any problems or
Even if fertilization is successful, a healthy pregnancy may not start. However, if the mother is young, quality
If a sperm and egg are selected, the success rate increases to 80 percent.
An experienced embryologist, a high quality microscope and equipment, a suitable laboratory environment
It is very important to achieve success with ICSI (microinjection). In our IVF treatments, the most
For good results, we also use the ICSI (microinjection) technique.

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